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Product Info Summary
| SKU: | M04354 |
|---|---|
| Size: | 50μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-Bassoon Bsn Antibody (Monoclonal, SAP7F407)
SKU/Catalog Number
M04354
Size
50μg/vial
Form
Liquid
Description
Boster Bio Anti-Bassoon Bsn Antibody (Monoclonal, SAP7F407) catalog # M04354. Tested in IP, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bassoon Bsn Antibody (Monoclonal, SAP7F407) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04354)
Host
Mouse
Contents
Each vial contains 50% glycerol and 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
SAP7F407
Isotype
Mouse IgG
Immunogen
Recombinant rat Bassoon.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M04354 is reactive to BSN in Mouse, Rat
Applications
M04354 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
416.469kDa
Background of Bassoon
Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500 - 1:1, 000, Mouse, Rat
IP, 2.5μg, Mouse, Rat
ICC, 1:400, Mouse, Rat
Validation Images & Assay Conditions
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Bassoon was detected in paraffin-embedded sections of rat brain cerebellar molecular layer using mouse anti-Bassoon Antigen Affinity purified monoclonal antibody (Catalog # M04354). The immunohistochemical section was developed using SABC method (Catalog # SA1021).
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Western blot analysis of Bassoon expression in MW marker (lane 1) and rat brain extract (lane 2). Bassoon at 400KD was detected using mouse anti-Bassoon Antigen Affinity purified monoclonal antibody (Catalog # M04354) at 1:500-1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For BSN (Source: Uniprot.org, NCBI)
Gene Name
BSN
Full Name
Protein bassoon
Weight
416.469kDa
Alternative Names
bassoon (presynaptic cytomatrix protein) BSN ZNF231 bassoon presynaptic cytomatrix protein protein bassoon|neuronal double zinc finger protein|zinc finger protein 231
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BSN, check out the BSN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BSN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bassoon Bsn Antibody (Monoclonal, SAP7F407) (M04354)
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No publications found for M04354
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Bassoon Bsn Antibody (Monoclonal, SAP7F407)
Question
We are currently using anti-Bassoon antibody (Monoclonal, SAP7F407) M04354 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Bassoon antibody (Monoclonal, SAP7F407) (M04354) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
I have a question about product M04354, anti-Bassoon antibody (Monoclonal, SAP7F407). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04354 anti-Bassoon antibody (Monoclonal, SAP7F407), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-17
Question
Is this M04354 anti-Bassoon antibody (Monoclonal, SAP7F407) reactive to the isotypes of BSN?
D. Krishna
Verified customer
Asked: 2013-09-18
Answer
The immunogen of M04354 anti-Bassoon antibody (Monoclonal, SAP7F407) is Recombinant rat Bassoon. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-18
